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Home / BIOL 103 Quizzes / BIOL 103 Module 43 Biotechnology
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BIOL 103 Module 43 Biotechnology

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Biotechnology Quiz

Question 1

What is the first step that would be required to produce human insulin in bacteria?

  • Back-translate the amino acid sequence to a DNA sequence
  • Determine the amino acid sequence of insulin.
  • Cut the synthetic gene and expression plasmid with restriction enzymes.
  • Ligate the DNA fragments.
  • Generate a synthetic gene using chemical synthesis of DNA.

Question 2

What is the second step that would be required to produce human insulin in bacteria?

  • Determine the amino acid sequence of insulin.
  • Generate a synthetic gene using chemical synthesis of DNA.
  • Back-translate the amino acid sequence to a DNA sequence
  • Ligate the DNA fragments.
  • Cut the synthetic gene and expression plasmid with restriction enzymes.

Question 3

What is the third step that would be required to produce human insulin in bacteria?

  • Determine the amino acid sequence of insulin.
  • Cut the synthetic gene and expression plasmid with restriction enzymes.
  • Generate a synthetic gene using chemical synthesis of DNA.
  • Ligate the DNA fragments.
  • Back-translate the amino acid sequence to a DNA sequence

Question 4

What is the fourth step that would be required to produce human insulin in bacteria?

  • Ligate the DNA fragments.
  • Back-translate the amino acid sequence to a DNA sequence
  • Generate a synthetic gene using chemical synthesis of DNA.
  • Determine the amino acid sequence of insulin.
  • Cut the synthetic gene and expression plasmid with restriction enzymes.

Question 5

What is the fifth step that would be required to produce human insulin in bacteria?

  • Back-translate the amino acid sequence to a DNA sequence
  • Generate a synthetic gene using chemical synthesis of DNA.
  • Cut the synthetic gene and expression plasmid with restriction enzymes.
  • Ligate the DNA fragments.
  • Determine the amino acid sequence of insulin.

Question 6

Examine the three base universal codon table below to answer this question.

The beginning part of the sequence of human insulin is Met-Ala-Leu-Trp. Which of the following sequences would be ideal for the production of human insulin in yeast?

  • ATG GCT TTA TGG
  • ATG GCG CTG TGG
  • ATG GCC CTT TGG

Question 7

The following is a Codon Table from a hypothetical organism that lacks the DNA base G and uses only two bases for its codons. Which of the following DNA sequences would represent a synthetic gene for the protein Met-His-Pro-Tyr?

  • AC CC CA TC
  • CT AC CC CA
  • CA CC AC CT AA
  • AC CC CA TC AA
  • AA CT AC CC CA

Question 8

The repressor in an expression plasmid is _______________________.

  • the binding site for RNA polymerase
  • none of the other selection
  • the DNA sequence that controls the production of mRNA
  • the sequence that directs the mRNA to the ribosome

Question 9

The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:

—GGATCCATCTGCAGATCT—-

—CCTAGGTAGACGTCTAGA—-

It contains a BamH1 site (G^GATCC) and a BglII site (A^GATCT). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end? Select all that apply.

  • EcoR1 (G^AATTC), followed by BamH1 (G^GATCC).
  • BamH1 (G^AATTC), followed by EcoR1 (G^AATTC).
  • BamH1 (G^GATCC), followed by BamH1 (G^GATCC).
  • BamH1 (G^GATCC), followed by BglII (A^GATCT).
  • BglII (A^GATCT), followed by BglII (A^GATCT).

Question 10

The following is the DNA sequence of the expression plasmid adjacent to the ribosome binding site:

-GAATTCGATATCAAGCTT

(EcoR1)(EcoRV) (HindIII)

You generated a synthetic gene for a human protein, but unfortunately you forgot to include the recognition sequences for EcoR1 at the beginning and for HindIII at the end of your synthetic gene, thus your gene looks like:

AGT——TAA

TCA——ATT

Given the following recognition sites: EcoR1 G^AATTC, EcoRV GAT^ATC, Hind III A^AGCTT, can you still insert your synthetic gene into the expression plasmid?

  • No
  • Yes

Biotechnology Quiz

Question 1

Examine the three base universal codon tables below to answer this question.

The beginning part of the sequence of human insulin is Met-Ala-Leu-Trp. Which of the following sequences would be ideal for the production of human insulin in yeast?

  • ATG GCC CTT TGG
  • ATG GCG CTG TGG
  • ATG GCT TTA TGG

Question 2

The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:

—GGATCCATCTGCAGATCT—-

—CCTAGGTAGACGTCTAGA—-

It contains a BamH1 site (G^GATCC) and a BglII site (A^GATCT). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end? Select all that apply.

  • EcoR1 (G^AATTC), followed by BamH1 (G^GATCC).
  • BamH1 (G^AATTC), followed by EcoR1 (G^AATTC).
  • BamH1 (G^GATCC), followed by BamH1 (G^GATCC).
  • BamH1 (G^GATCC), followed by BglII (A^GATCT).
  • BglII (A^GATCT), followed by BglII (A^GATCT).

Question 3

A segment of DNA containing the sequence -AAGCTT- is cut with an enzyme, Oli1 (recognizes AAGCTT). DNA treated with which of the following enzymes could be ligated to this DNA? Select the best answer.

  • Oli2, recognizing AAGCTT.
  • Oli3, recognizing TAGCT^A.
  • Oli4, recognizing ATC^GAT.
  • none of the above.

Question 4

The following is a Codon Table from a hypothetical organism that lacks the DNA base G and uses only two bases for its codons. Which of the following DNA sequences would represent a synthetic gene for the protein Met-His-Pro-Tyr?

  • AC CC CA TC AA
  • CT AC CC CA
  • AC CC CA TC
  • CA CC AC CT AA
  • AA CT AC CC CA

Question 5

The repressor in an expression plasmid is _______________________.

  • the sequence that directs the mRNA to the ribosome
  • the DNA sequence that controls the production of mRNA
  • none of the other selection
  • the binding site for RNA polymerase

Question 6

The following is the DNA sequence of the expression plasmid adjacent to the ribosome binding site:

-GAATTCGATATCAAGCTT

(EcoR1)(EcoRV) (HindIII)

You generated a synthetic gene for a human protein, but unfortunately you forgot to include the recognition sequences for EcoR1 at the beginning and for HindIII at the end of your synthetic gene, thus your gene looks like:

AGT——TAA

TCA——ATT

Given the following recognition sites: EcoR1 G^AATTC, EcoRV GAT^ATC, Hind III A^AGCTT, can you still insert your synthetic gene into the expression plasmid?

  • No
  • Yes

 

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